OPERATION
Carl Zeiss Illumination and contrasting techniques Axiovert 200
3-36 B 40-080 e 03/01
(2) Transmitted-light DIC with analyzer slider ±
±±
±30° (de SENARMONT)
When the analyzer slider ±30° is used, the DIC slider must be brought to the center position first.
• Swing in the polarizer and bring analyzer ±30° (3-20/5) in 0°-position (dark position) (polarizer and
analyzer are crossed).
• Swing out the DIC prism on the condenser turret (use brightfield or phase contrast position, for
example).
• Remove one eyepiece and replace it with the centering telescope (or switch on Bertrand lens on the
phototube).
• If the field is viewed using the centering telescope (or Bertrand lens), a diagonal black line of the DIC
slider becomes visible (from the left top to the right bottom).
• Adjust the knurled screw on the DIC slider to move the diagonal black line to the center of the field of
view.
• Remove the centering telescope and insert the eyepiece again (or switch off Bertrand lens).
• Swing in DIC position on the condenser.
• Place a specimen on the stage.
• Use the setting wheel to turn the analyzer out of the 0°-position and to set the optimum contrast in
this way.
☞
Since the DIC technique uses polarized light, it is impaired if birefringent components, e.g. foils
sometimes used with histological sections, are positioned between polarizer and analyzer. The
same applies to Plexiglas culture chambers if the chamber bottom is made of plastic. In such
cases, it is recommended to use chambers with glass bottoms to avoid the loss of optical
performance.
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