14 Purification Solution - Developer's Guide
2
Preparing Default Purification Methods
General Method Settings
UV (DAD, MWD,VWD)
UIB II (G1390B)
• If an auxiliary detector is connected via the UIB II box, set all related
parameters in the UIB II method.
• If there is no detector connected via UIB II box, clear both Analog In check
boxes in Advanced > Analog In Port Settings
• Set Stoptime to As Pump/Injector
Fraction Collectors (G1364B, G1364E, G7159B, G7166A and FCC
*
)
*
FCC is a Fraction Collector Cluster of two or more modules.
• G1364E, G7159B, G7166A and FCC: Set Collection Behavior to Disable Fraction
Collection
• Set Stoptime to As Pump/Injector
Wavelength DAD and MWD
Set Signal AWavelength to 263 nm, Bandwidth: 4 nm, clear
Reference wavelength.
VWD
Set Signal to 263 nm.
Peakwidth DAD and MWD
20 Hz (> 0.013 min)
VWD
40 Hz (> 0.013 min)
Advanced > Spectrum
(optional for DAD)
Store: All, Range from: 200 nm to 400 nm, Step: 2 nm
Autobalance Mark Prerun.
Lamps on required for
acquisition
(DAD and MWD)
Mark UV Lamp.
Miscellaneous
VWD
Mark Lamps on required for acquisition.
Stoptime Select As Pump/Injector
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