Purification Solution - Developer's Guide 17
Preparing Default Purification Methods
2
Analytical Method Settings
Dual Loop Autosampler (G2258A)
Valve 2/10 (G1170A and G4730A)
UIB II (G1390B)
If the Active Splitter is connected to the external contacts of the UIB II:
• Timetable: Empty
• External Contacts: Contact A: mark Closed
Injection Loop Lower
Injection mode Depends on installed loop volume and injection volume.
• Loop equal to injected volume: set Full loop with overfill
factor 5, disable Plug Settings
• Loop larger than injected volume: set Partial loop filling and
set Plug Settings to maximum Plug volume:
• Mark Draw Plug before and after the sample
• Plug Volume: set the volume later in the method based on
injection volume: Plug volume = ½[(Loop volume) –
(Injection volume)]
• Draw Plug from: select Location, specify vial location
The application of the Plug removes up to 27 µL of the
purge solvent from the lower loop that is introduced by the
seat capillary in front of the loop, which is purged after
each run. The purge solvent has typically high elution
strength by design; therefore, the removal of this solvent
from the lower injection loop by the Plug feature is highly
recommended.
Injector Cleaning Rinse volume to 15
Advanced Draw speed and Eject speed to 1000.0 µL/min.
Position Use valve position: set analytical position
Expected ERI Mode No mode check
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