Purification Solution - Developer's Guide 29
Setting Up an Analytical and Preparative System
3
Setting up an analytical system
runs. The preparative flow rate can be calculated from the analytical
flow considering the different column geometry in order to maintain the
same chromatographic conditions. It is also possible to adapt the
analytical flow rate according to a given preparative flow rate from the
selected preparative system in the right panel of the Setup Systems
Parameters dialog box.
• In the Detectors section, select the relevant detectors from the available
detectors in the instrument configuration. The system schematics
graphic corresponds to the selected detectors.
4 In the Sampler tab:
• The tab is present only for flow-through samplers such as the G7157A
1260 Infinity II Prep Autosampler. There, the volumes of the sample loop
and seat capillary contribute to the time that the sample needs to reach
the column. If the analytical system is separate from the preparative
system, such volumes are read from the method as specified in the Pumps
& Detectors tab. For combined instruments, the volumes must be
imported from the current instrument configuration. Previously created
analytical system settings may refer to a different sample loop or seat
capillary and then will be displayed with a warning sign.
5 In the Delay Volumes tab:
• Select the tubing by selecting a system type, and provide the initial
pressure.
If you select a custom system type, no initial pressure is required, but
you need to determine the tubing volumes. For details about determining
delay volumes and times, see Characterizing the delay volumes
(“Characterizing the delay volumes: Mixing point to UV detector and
Column to UV detector” on page 48).
• Specify the delay times of an MSD or an auxiliary detector to the UV
detector, if such detectors are selected in the Pumps & Detectors tab.
6 Switch to the Column tab and provide details of the analytical column.
7 Click Save System to save the analytical system parameters.
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