Purification Solution - Developer's Guide 61
Calibration Procedures
6
Characterizing the delay volumes: Mixing point to UV detector and Column to UV detector
• Zoom in to the signal after the elution of acetone and note the
absorbance approximately 1 min after the signal rises as A
2
.
• Calculate A
1/2
= (A
1
+ A
2
)/2
3 Zoom in several times to the curve around A
1/2
and note the elution time
with a precision of three decimal places.
The signal after the elution of acetone is typically not completely stable, so it is sufficient
to have only an approximate reading.
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