90 Purification Solution - Developer's Guide
7
Checkout Procedure
UV-only Workflow: Focused gradient in the Purification software
• Review the analytical and preparative flows in the Pumps and Detectors
tab if the target flows are set.
• Analytical Run
• Click Browse and select the acquired analytical sequence
(Checkout_Sample)
• Preparative Run
• Select the Preparative base method (Prep_Purification)
• Keep Review analytical results before purification marked.
• Set the preparative vial Location.
• Set the Inj. volume to 500 μL.
• Fraction collection tab (in Preparative Run)
• UV detector: select Trigger mode > Threshold.
• Gradient tab (in Scale up): keep the Initial concentration: 2 % check box
marked.
7 Click Run.
8 Set the parameters in Analytical Results:
• Set a suitable threshold in the Fraction collection tab:
• UV detector: set Threshold to 5 or adjust the value to achieve a
threshold of about 5 % of the peak height in the preparative run.
9 Review the Analytical Results (check the settings and the target peak):
• Select the first major peak (caffeine) in the chromatogram and click
Assign As Target above the chromatogram.
10 Click Run again to submit the preparative task to the ChemStation run
queue.
11 When the preparative task is completed, check in Preparative Results that the
correct peak was focused and that it is among collected fractions.
12 Create a new task:
• Click Add Task.
• Select
Add clone of currently selected task and click OK.
13 Review the Analytical Results:
• Select the third major peak (Ethyl 4-hydroxybenzoate) in the
chromatogram and click Assign As Target above the chromatogram.
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