24 1260 Infinity Micro Degasser Service Manual
3 Installing the Micro Vacuum Degasser
Operational Hints for the Micro Vacuum Degasser
Operational Hints for the Micro Vacuum Degasser
General Priming Instructions
Before using a new degasser or new tubings for the first time:
1 Prime all tubings with at least 5 ml of iso- propanol no matter whether
the channels will be used with organic mobile phase or with water.
If you are changing to a solvent that is immiscible with the solvent
currently in the tubing continue as follows:
2 Replace the current solvent with iso- propanol, if current solvent is
organic or with water, if current solvent is an inorganic buffer or
contains salt.
Priming the Degasser
The micro vacuum degasser can be primed by drawing solvent through the
degasser by pumping with the connected pump.
For priming the micro vacuum degasser using the pump the following
is recommended:
1 Connect all tubings to and from degasser and pump.
2 Open the pump’s purge valve to waste.
3 Pump at low to moderate speed, until the solvent reaches into the
pump. Pumping at higher flow rates will stress the system and might
result in a failure to prime the degasser with the pump.
When opening capillary or tube fittings, solvents may leak out.
The handling of toxic and hazardous solvents and reagents can carry health risks.
➔ Observe appropriate safety procedures (for example, wear goggles, safety gloves
and protective clothing) as described in the material handling and safety data sheet
supplied by the solvent vendor, especially when toxic or hazardous solvents are
used.
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