30 1260 Infinity Micro Degasser Service Manual
4 Using the Micro Vacuum Degasser
When to Use a Micro Vacuum Degasser?
When to Use a Micro Vacuum Degasser?
For capillary LC application with our Agilent 1260 Infinity Capillary and
Nano LC System
For high- pressure mixing pumps (binary and binary SL) with low flow
rates or when you have following conditions:
• if your detector is used with maximum sensitivity in the low UV
wavelength range,
• if your application requires optimum injection precision,
• if your application requires highest retention time reproducibility
(mandatory at flow rates below 0.5 ml/min),
• if your sample or detection is sensitive to dissolved oxygen in the
mobile phase (degradation),
• with a fluorescence detector,
• with an LC- MS- detector.
Generally a degasser should be used when negative effects due to
dissolved gas in the mobile phase exceed the limits that are acceptable for
the user. Negative effects that can be caused by dissolved gas are:
• Unstable flow due to unstable pumping conditions. This may result in a
high ripple or high standard deviations of peak retention times and
peak areas especially at low flow rates.
• Baseline noise on detectors that are sensitive to changes in the
refractive index,
• sample degradation,
• fluorescence Quenching due to dissolved oxygen,
Unspecified Conditions
Operating the instrumentation under conditions other than its intended use might
result in a potential safety hazard or might damage the instrumentation.
➔ Never operate your instrumentation under conditions other than those specified by
the vendor.
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