1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual 39
Using the Module
3
Configuration and Operation of the Fraction Collector
Recommended Calibrants
Table 5 Recommended calibrants (P/N) for Agilent Fraction Collectors using
ChemStation
Fraction Collector type UV UV and MSD
G1364 B/C/D/E/F, G7159B and
G7166A
5190-8223 5190-8223
G5664 A/B G1946-85020 or
5190-8223
5190-6887 or
5190-8223
Table 6 Calibrants and recommended wavelength settings for UV-based delay calibration
Part No. Name Dye Wavelength
5190-8223 Agilent delay and checkout calibrant Patent Blue VF 600 nm
5190-6887 Prep LC Standard #2 Thionin acetate 600 nm
G1946-85020 Delay Sensor Calibration Sample Kit FD&C Green 3 (Caffeine for MSD) 600 nm
Table 7 Calibrants and target masses for MSD delay calibration
Part No. Name Dye Target Mass
5190-8223 Agilent delay and checkout
calibrant
Patent Blue VF m/z 543.1 (neg)
m/z 545.1 (pos)
m/z 589.1 (pos, Na adduct)
5190-6887 Prep LC Standard #2 Thionin acetate m/z 228.1 (without acetate)
G1946-85020 Delay Sensor Calibration Sample
Kit
FD&C Green 3 (for UV and
fraction delay sensor)
Caffeine (for MSD)
m/z 195.1 (pos)
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