18 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Delay volumes and delay calibration
Performing a delay calibration with an UV detector
1 Place a vial containing the Delay Sensor Calibrant (Part No. G1946-85020)
in position 1 of the Autosampler.
2 Remove the installed column and connect the capillaries with a
zero-dead-volume connector.
3 Connect a bottle of water to Channel A.
4 Install the Delay Calibration Adapter G1364-87301 by attaching it to the
needle carrier assembly (G1364B preparative scale only).
5 Switch to Diagnosis View (if necessary).
6 Open the AFC Delay Volume Calibration status window (see Figure 4) from
the Fraction Collector sub-menu of the Maintenance menu:
Maintenance –> Fraction Collector –> Delay Volume Calibration
The method to be used for the delay volume calibration is displayed
(DGCALAS.M is the default calibration method for the analytical scale
fraction collector and DGCALPS.M is the default method for the preparative
fraction collector); if you want to change to a different method, click the
Change Method button and select a new method. See also “Making Your
Own Calibration Method” on page 26
The Info field shows the current status of the delay volume calibration, and
gives instructions and warnings.
Figure 4 AFC Delay Volume Calibration window
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