40 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Special Applications
Semi-preparative operation
The analytical scale fraction collector G1364C can be modified for
semi-preparative operation. This allows to use of external collection vessels
through funnels at high flow rates above 10 ml/min. The maximum flow rate
depends on the viscosity of the solvent.
In order to modify the analytical fraction collector the following parts have to
be installed:
• 20 mm needle assembly (G1364-87202)
• preparative tubing kit (G1364-68711)
• internal tray preparative scale (G1364-63113)
The procedures to exchange these parts are described in“Replacing the
Inlet/Waste Tubings” on page 59, “Replacing the Valve to Needle Tubing” on
page 63, “Exchanging the Analytical Needle Assembly” on page 68, and
“Exchanging the Internal Tray” on page 74.
In addition the wellplate adapter has to be removed for operation with the
shorter needle.
In the ChemStation Software the new configuration has to be specified in
order to consider the new volumes for the delay calibration.In the Configuration
dialog box of the fraction collector select More. Then change Inner Capillary
Diameter to 0.8 mm as well as the Needle Type to semi-prep needle the as
displayed in Figure 19 on page 41. For further information on delay volumes
and delay volume calibration see “Delay volumes and delay calibration” on
page 15.
CAUTION
With each start of a sequence run the recovery will start at the same positions. In order
to avoid contamination the vessels that contain the recovery fractions have to be
exchanged.
CAUTION
The number of recovery locations automatically defines the maximum number of
injections. When using the standard tray for two well plates and 10 funnels, only ten
injections per sequence are executed.
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