22 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Delay volumes and delay calibration
Performing a delay calibration with an MSD
1 Place a vial with the Delay Sensor calibrant (Part No. G1946-85020) in
position 1 of the Autosampler.
2 Remove the installed column and connect the capillaries with a
zero-dead-volume connector or a mixer (Part No. 79835-87330).
3 Connect a bottle of water to Channel A of the main pump.
4 Install the Delay Calibration Adapter G1364-87301 by attaching it to the
needle carrier assembly (G1364B preparative scale only).
5 Connect a bottle of water with 0.1% acetic acid or ammonium formate to the
make-up pump.
6 Load the method DGCALAS.M (analytical scale) or DGCALPS.M
(preparative scale) and adjust the flow in the main pump to the flow you
will be using for the analysis.
7 Set the flow of the make-up pump to the flow rate you will be using for your
analysis.
8 Set the active splitter to a setting of 3.
9 Save the method to a new name.
10 Go to Diagnostics menu; select Delay volume Calibration from the Fraction
Collector Sub-menu within the Maintenance menu.
11 Press the Change method... button to select the method you created. The
new method name appears on the info line.
12 Start the active splitter.
13 Press the Continue button to execute the Delay Volume Calibration
The selected method is loaded and the Delay Calibration Sample Injected.
14 When the Calibration Run is finished, press OK.
15 Examine the value for the MSD Calculated Delay time.
CAUTION
Unlike for UV detectors, the delay calibration for the MSD needs to be performed
whenever the flow rate is changed.
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