Agilent 1100 Series Fraction Collectors User’s Guide 23
Configuration and Operation of the Fraction Collector 1
Delay volumes and delay calibration
The delay time should be at least 5 seconds but may, in fact, be negative (see
Figure 7).
16 If the measured delay time is greater than 5 seconds, you can skip to
step 21, otherwise, continue with step 17.
17 Determine how much additional time is needed to create a 5-second delay.
For example, if the time displayed is -1 sec, a six-second delay is needed.
18 Using the main pump flow rate, determine the desired additional delay
volume. For example, at 3 ml/min and a six-second delay, an additional
3.0 x 6/60 or 0.3 ml is required.
19 Cut off a sufficient piece of the supplied ETFE tubing (Part No.
G1968-60500) to provide the needed volume. The tubing's volume is 8.1
µl/cm. In this case, 37 cm would be required.
20 Add the tubing between the splitter and the fraction collector or, if multiple
fraction collectors are installed, between the splitter and the G1160A valve.
Figure 7 Delay Volume Calibration results
CAUTION
If the measured delay time is significantly greater than 5 seconds, you should continue
with the calibration at step 16 to avoid losing fractions at the end of the run.
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