38 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Special Applications
Special Applications
Pooling
We define Pooling as collecting fractions from multiple injections into the
same fraction locations. In the Agilent Chemstation, there are two ways to
initiate pooling:
• If you want to collect multiple injections from the one sample vial or sample
well into the same fraction location, specify a Fract. Start and multiple
injections in the Sequence Table.
• In case you want to collect multiple injections from the several sample vials
or sample wells into the same fraction location, specify the same Fract. Start
for multiple samples in the Sequence Table. An example is displayed
inFigure 17 on page 38. In this sequence the Sample 1 will be injected from
Plate 1 Position A1 of the 1100 Series wellplate sampler and fraction
collection will start at Plate 1 Position A1 of the fraction collector.
For the next Sample 2, which is injected from a different location in the
wellplate sampler, the fraction collection will start again at Plate 1 Position
A1 of the fraction collector.
Figure 17 Pooling Sequence
CAUTION
When pooling fractions, overfill protection no longer exists. It is the user's
responsibility to make sure that all fraction collection locations are large enough to
completely collect all pooled fractions. If a fraction collection location is overfilled, an
error message occurs and the pump is shut off
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