Introduction
NovoCyte Quanteon Flow Cytometer Operator's Guide 31
Figure 1. Longpass Filter and Shortpass Filter
The bandpass filters are usually placed at a 90° angle relative to the incident light
and regulate the incident light. The transmitted light travels in the same direction
as the incident light (λ2), whereas the reflected light is reflected in a 180° angle
relative to the incident light (λ1 and λ3) (Figure 2).
Figure 2. Bandpass Filter
• Longpass Filters
Longpass (LP) filters pass wavelength longer than the cutoff wavelength. For
example, a 505 nm LP filter allows wavelengths longer than 505 nm to pass
through it and either absorbs or reflects wavelengths shorter than 505 nm as
shown in Figure 3.