3Getting Started
Sample Preparation and Loading
20 Agilent SureCycler 8800 Setup and User’s Guide
Sample Preparation and Loading
To prevent damage to the block and the heated lid, use only
temperature- stable sample tubes and plates. Unsuitable tubes and plates
can become damaged during cycling. See “Agilent Plasticware and
Consumables” on page 21 for a list of recommended plasticware.
To prepare samples
Keep the following guidelines in mind when preparing samples in reaction
plates and tubes:
• Place caps on tubes before loading samples into the thermal block.
• For best results, just before loading
the
samples into the thermal block,
spin them briefly in a centrifuge.
• If using sealing film to cover a reaction plate, use a compression mat
dur
ing cycling to ensure the film stays firmly adhered to the plate. See
“Agilent Plasticware and Consumables” on page 21 for ordering
information on a compression mat.
To load samples
The instrument can be loaded with individual PCR tubes or strip tubes or,
depending upon the thermal block installed, one 96- well PCR plate or one
384- well PCR plate.
1 Lift the lid of the thermal block by pressing firmly on the button on the
top o
f the instrument to unlatch the lid.
2 Put your plate or tubes on the block and check that they are correctly
positioned.
On the 96- well
block, seat the tubes in the round
holes on the thermal
block. On the 384- well block, seat the tubes in the larger round holes.
3 Close the lid until it latches into place.
Danger of Burns: The thermal block, sample tubes and plates may reach
temperatures as high as 100°C. Keep hands away until temperature is 30°C or less.
To Next Page
Loading...
Need help?
General
