PCR Programs 4
End Cycle
Agilent SureCycler 8800 Setup and User’s Guide 47
Temperature
Increment
Enter the desired increase or decrease in the processing temperature with
each cy
cle. Press the +/– button to toggle between positive and negative
numbers. Negative numbers (e.g. –2°C) indicate an incremental decrease in
temperature with each cycle.
Time Increment: Enter the desired increase in the Hold
For
time with each cycle. The units
for this field are seconds/cycle.
End Cycle
To have a valid program, the steps of a cycle need to be contained between a Start Cycle
and an End Cycle command.
Insert this program step to terminate a PCR cycle. Put this step after a
series of steps that are to be repeated for several cycles.
Gradient Step
A Gradient Step is similar to a Temperature Step, but in a Gradient Step
the thermal block holds a temperature gradient (rather than a single
temperature across the entire block) for a specified length of time.
The Gradient Step is useful for optimizing the annealing temperature for
your particular primer/template system.
The fields in the Increments per Cycle section are used to set temperature and time
increments for the temperature step during cycling. The use of increments is an optional
feature. Leave these fields blank if you do not want to add increments.
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