Agilent Technologies SureCycler 8800 - Cycle Wizard

4PCR Programs
Cycle Wizard
44 Agilent SureCycler 8800 Setup and User’s Guide
Cycle Wizard
The Cycle Wizard command can be used to create the steps of the
program that are to be cycled multiple times. Using the Cycle Wizard to
create cycled steps replaces the need to manually add Start Cycle and End
Cycle commands. These steps will automatically be added to the program
by the wizard.
Denaturation
Step
Enter the denaturation temperature in
°C
(left field) and denaturation
time in hours : minutes : seconds (right field).
Annealing Step Enter the annealing temperature in °C (lef
t f
ield) and annealing time in
hours : minutes : seconds (right field).
Elongation Step If your PCR protocol uses 3- step cycling with separate annealing and
elon
gation st
eps (rather than a 2- step cycling protocol with a combined
annealing/elongation step), mark the check box labeled Use 3rd step.
Enter the elongation temperature in °C (left field) and elongation time in
hours : minutes : seconds (right field).
Cycles
Enter the number of cycles.