PCR Programs 4
If condensation is found in tubes during a run
Agilent SureCycler 8800 Setup and User’s Guide 59
If condensation is found in tubes during a run
Condensation will appear naturally at the end of a run and has no
deleterious effects.
✔ Spin the liquid back to the bottom of the tube and continue with the
post PCR
st
eps.
The hot top is disabled.
✔ Enable the hot top using the instructions in “Hot Top Setting” on
page 32.
If the sealed microplate loses sample volume
✔ Check that the microplate/sealing system is of good quality.
✔ Ensure a compression mat is used during the PCR run to keep the
sea
ling film adhered to the plate.
If USB devices including memory stick and keyboard not
recognized
✔ Reboot the cycler.
If cycler does not turn on
✔ Check that the power is on at supply and the power cord is connected.
✔ Check fuses at rear of instrument above power inlet.
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