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Agilent Technologies SureCycler 8800 User Manual

Agilent Technologies SureCycler 8800
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Agilent Technologies
SureCycler 8800
Setup and User’s Guide
Version A, March 2011

Table of Contents

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Agilent Technologies SureCycler 8800 Specifications

General IconGeneral
Block Capacity96-well
Temperature Accuracy±0.25°C
Temperature Uniformity±0.4 °C at 55 °C
Weight10 kg (22 lb)
DisplayLCD
InterfaceRS-232
Dimensions26 cm x 47 cm x 24 cm
Power Requirements100–240 V, 50/60 Hz
Control TypeMicroprocessor controlled
Sample Capacity96 x 0.2 mL tubes

Summary

Before You Begin

Materials Provided

Lists all parts included with the SureCycler 8800 instrument.

Safety Precautions

Details electrical, fluid, burn, and environmental safety guidelines for instrument use.

Indicator LED Light

Explains the status codes indicated by the instrument's LED light.

Overview of SureCycler 8800

Introduces the SureCycler 8800's capabilities and software interface.

SureCycler 8800 Technical Support

Provides contact information for technical assistance via email, web, and phone.

Installation Guide

Instrument Installation Steps

Step-by-step guide for setting up the SureCycler 8800 instrument.

Thermal Block Exchange

Instructions for removing and installing interchangeable thermal block units.

Getting Started with SureCycler 8800

Sample Preparation and Loading

Guidelines for preparing and loading samples into the SureCycler 8800.

Agilent Plasticware and Consumables

Lists compatible PCR plates, tubes, and sealing films for the instrument.

Starting Up the Software

Instructions for powering on and interacting with the instrument's software interface.

User Accounts Management

Explains access levels, logging in, and managing user accounts for the instrument.

Network Settings and Remote Access

Details on configuring network settings and accessing the cycler remotely.

Cycler Settings Configuration

How to configure instrument settings like hot top, name, and time/date.

PCR Programs

Creating New PCR Programs

Methods for creating new PCR programs manually or using the Program Wizard.

Tips for 384-well PCR Programs

Advice for optimizing PCR program creation on the 384-well block.

Program Steps in Commands List

Explanation of various program steps like Hot Start, Cycle Wizard, etc.

Using Preloaded PCR Programs

How to use and modify pre-existing PCR programs.

Editing PCR Programs

Procedures for modifying existing PCR program parameters and steps.

Running a PCR Program

Steps to start, stop, or pause a PCR program execution.

Running an Incubation

How to set up and run single-temperature incubations on the instrument.

Viewing PCR Reports

How to access, view, and delete GLP reports generated by PCR programs.

Troubleshooting PCR Programs

Common issues and recommended solutions for the SureCycler 8800.

Maintenance and Updates

Instrument Maintenance

Procedures for cleaning the instrument and replacing a fuse.

Software Updates

Instructions for downloading and installing new software versions for the instrument.

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