4PCR Programs
Troubleshooting
58 Agilent SureCycler 8800 Setup and User’s Guide
Troubleshooting
This section describes potential problems and recommended actions.
If PCR yield is low when running small reaction volumes with the
Standard ramp rate
✔ If you are using sealing films/mats, shorten the duration of the
denaturation step during cycling. Denaturation times as short as 3–5
seconds may be appropriate. Alternatively, switch to using capped
reaction tubes.
✔ Increase the reaction volume to 50 μl.
✔ Try switching to the Fast ramp rate.
If no actual temperature is displayed during the run
The thermal block unit may not be correctly seated on the instrument.
✔ Check that the unit is installed correctly and reboot the cycler.
If the program does not start
The thermal block unit may not be correctly seated on the instrument.
✔ Check that the unit is installed correctly and reboot the cycler.
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