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Agilent Technologies 4150 TapeStation system User Manual

Agilent Technologies 4150 TapeStation system
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Agilent Technologies
Agilent 4150 TapeStation
System
System Manual

Table of Contents

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Agilent Technologies 4150 TapeStation system Specifications

General IconGeneral
Instrument TypeAutomated Electrophoresis System
Input Sample TypeDNA, RNA
Detection MethodFluorescence
Software CompatibilityWindows
ConnectivityUSB, Ethernet
Weight12 kg
Analysis Time1-2 minutes per sample
Sample Volume2 µL
Data AnalysisAutomated
Data OutputElectropherograms, tables, reports
SoftwareAgilent TapeStation Analysis Software
Operating SystemWindows
Power Requirements100-240 V AC, 50/60 Hz

Summary

Safety

Symbols

Explanation of safety symbols used in the manual.

Safety Messages

Definitions and explanations of WARNING and CAUTION messages.

General Safety Information

General safety precautions to be observed during operation and service.

Legal and Regulatory

Sound Emission

Manufacturer's declaration regarding sound pressure emission levels.

Waste Electrical and Electronic Equipment (WEEE) Directive

Information on product compliance with WEEE directive for disposal.

Product Description

Intended use of the 4150 TapeStation System

Specifies the intended applications and usage of the system.

Overview of the System and ScreenTape Architecture

Provides a high-level view of the instrument and its components.

Physical Specification

Details the physical dimensions, weight, and environmental specifications.

Installation

Site Requirements

General safety precautions and requirements for the installation environment.

Bench Space Required

Information on the physical space needed for the instrument setup.

Unpacking

Step-by-step instructions for safely removing the instrument from its packaging.

Initial Setup of the System

Procedures for the initial power-up and connection of the instrument.

Prepare the Working Area

Steps to prepare the instrument's workspace for operation.

TapeStation Software

TapeStation Controller Software

Overview of the software for instrument control and data acquisition.

TapeStation Analysis Software

Description of the software used for data analysis and reporting.

Agilent Information Center

Access to documentation, guides, and videos for TapeStation system.

Operating Instructions

Sample Preparation

Guidelines for preparing samples according to specific assay requirements.

Preparing the Instrument

Steps to prepare the instrument before starting an analysis.

Starting the Analysis

Procedures for initiating and running an analysis on the system.

Analysis End

Steps taken after the analysis is complete, including instrument reset.

Follow Up

Post-run procedures for gathering used materials and preparing for the next run.

Good Measurement Practices

Use of Correct Tools

Guidelines on using appropriate tools for accurate results.

Tools and Handling

Best practices for handling tools and samples to prevent contamination.

Good Pipetting Practice

Techniques for consistent and accurate pipetting.

Reagents and Reagent Mixes

Proper handling, storage, and mixing of reagents for optimal performance.

ScreenTape Devices

Handling, storage, and preparation of ScreenTape devices.

Sample Preparation

Procedures and considerations for preparing samples for analysis.

Ladder Considerations

Guidelines for using ladders for accurate sizing and quantification.

Use the Correct Protocol

Importance of following specific assay protocols for accurate results.

Mixing Recommendations

Recommended methods for mixing samples and reagents.

Centrifugation Recommendations

Procedures for centrifuging samples to remove bubbles and collect liquid.

RNA Assays

Specific recommendations and procedures for RNA analysis.

Peak Integration

Methods for correctly integrating peaks for accurate quantification and sizing.

Instrument Diagnosis

Instrument Diagnosis

Overview of the system diagnostics for maintenance and troubleshooting.

Tests

Description of available test groups for system verification and troubleshooting.

Software Installation Verification

Checks computer specifications and verifies correct component installation.

System Verification

Tests to confirm the functionality of various system components.

Hardware Troubleshooting

Comprehensive tests for diagnosing hardware performance issues.

TapeStation Test Tape

Information on the test tape used for hardware diagnosis.

Executing System Verification Tests

Step-by-step guide to running system verification tests.

Reviewing Test Results

How to print, review, and save test execution results.

Maintenance

Maintenance Information

Details on needle pierce counters and maintenance schedules.

Exchange the Piercing Needle

Procedure for replacing the piercing needle.

Electrode Cartridge

Information about the electrode cartridge and its function.

Cleaning the Instrument

Instructions for cleaning the instrument and its parts.

Preparing the Instrument for Transportation

Steps to prepare the instrument for shipping or moving.

Boxing for Transportation

Instructions for packaging the instrument for safe transport.

Assembling Nest Covers

Procedures for correctly re-installing nest covers.

Insertion of the Lower Nest Cover

Instructions for correctly inserting or re-installing the lower nest cover.

Troubleshooting

Software Messages

Common error messages and their causes.

Troubleshooting DNA Applications

Guidance for resolving issues specific to DNA sample analysis.

Troubleshooting RNA Applications

Guidance for resolving issues specific to RNA sample analysis.

Instrument Communication

Best practices to mitigate communication issues between instrument and laptop.

Collecting Troubleshooting Information

Steps to gather diagnostic data for technical support.

Appendix

Accessories Shipment List

List of accessories shipped with the Agilent 4150 TapeStation system.

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