Agilent 4150 TapeStation System Manual 185
Troubleshooting
10
Troubleshooting DNA Applications
Sizing in Assays with Mandatory Ladder
No sizing and quantification results can be obtained in the Genomic DNA,
D5000 and D5000 High Sensitivity assays if the correct number of ladder
peaks was not assigned.
Missing Marker Peaks
Table 8 DNA - Possible causes of missing marker peaks in order of probability
Root cause Explanation Solution
Sample
concentration
outside
recommended range
for application
The majority of the dye binds to the sample so
that the signal from the marker is too weak to
be detected if the sample is too concentrated.
Dilute your sample until it is within the
recommended range for the application as
stated in the assay Quick Guide; then prepare a
new run (Ta bl e 3 on page 64).
Insufficient migration Marker peak may not be completely resolved
from a sample peak or is not at an expected run
distance.
To check migration true distance, unalign the
gel image using the Aligned button. See
“Unexpected Migration Profile” on page 188,
“Maximum Buffer Concentration in Sample” on
page 110, and “Quantitative range” on
page 107.
Blank lane See the Agilent Information Center for more
information.
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