Agilent 4150 TapeStation System Manual 93
Good Measurement Practices
7
Use the Correct Protocol
Use the Correct Protocol
• Each ScreenTape type is designed for use with its corresponding Reagent
kit. Reagent kits are not interchangeable between standard and high
sensitivity assays due to different dye and marker concentrations.
• Ensure that sample and sample buffer volumes from the correct protocol
are followed. The assays require a specific sample to buffer ratio. If not
followed correctly, sizing may be impaired due to altered run distance as
well as quantification may be affected due to altered dye and marker
concentration.
• Ensure that the reagents are used with the corresponding ScreenTape type.
• It is important to choose the correct assay based on the concentration of
the sample. Using sample concentrations outside the specified quantitative
ranges will lead to inaccurate quantification.
• For correct volumes and quantitative range, please refer to the appropriate
Quick Guide (“Sample Preparation” on page 64).
Figure 20
Effect of correct (A1) and incorrect consumables/protocol (B1 – D1) on the D1000
Ladder peak intensity and Marker assignment in the D1000 ScreenTape assay.
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