104 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
Peak Maxima Versus Average Molecular Weight Sizing and Molarity
Peak Maxima Versus Average Molecular Weight Sizing and
Molarity
Within the TapeStation Analysis software, results can be accessed in both
electropherogram and region mode/view. The sizing information presented in
these modes is different.
Electropherogram view is designed for use with discrete peaks, and the default
size reported is that of the highest point of the peak.
Region view calculates data over a whole smear or region (for example NGS
libraries), and reports size as that of the center of the regions’ mass. This gives
the user an idea of the distribution of sizes within that sample.
The same holds true for quantification results, especially molarity as this is
determined from both size and quantity.
Errors in sizing and quantification result in erroneous molarity calculations.
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