94 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
Mixing Recommendations
Mixing Recommendations
Use a vortexer which is designed for mixing tube strips, or 96-well sample
plates respectively. The IKA MS3 vortexer is recommended for all ScreenTape
assays. TapeStation systems are supplied with an optional IKA MS3 vortexer
which includes a 96-well sample plate adaptor suitable for both 96-well PCR
plates and tube strips.
• Close tube strips with tube strip caps and seal 96-well sample plates with
96-well plate foil seal.
• Set the IKA MS3 vortexer to 2000 rpm.
• Vortex for 1 min by pressing the start button.
Figure 21 IKA MS3 vortexer
If no IKA MS3 vortexer is available, please ensure thorough manual vortex
mixing — 1 min on maximum speed.
• Sample and sample buffer must be vortex mixed according to protocol,
followed by centrifugation to remove any bubbles.
• Insufficient mixing can cause discrepancies in quantification.
• Poor mixing dramatically affects the reported sample concentration.
Following the recommended procedure is the best way to attain accurate
quantification results.
The effects of insufficient mixing were investigated using the D1000
ScreenTape assay, and results are presented in the figures and table below.
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