Agilent 4150 TapeStation System Manual 83
Good Measurement Practices
7
Use of Correct Tools
Use of Correct Tools
• Use calibrated pipettes which are suitable for the volume required.
• Ensure correct pipetting technique, so that volumes are precise, and that
the concentrations can be calculated correctly.
• Use a vortexer that is designed for mixing 8-way tube strips, or 96-well
sample plates respectively.
• For more details, please see “Mixing Recommendations” on page 94.
• Use only the correct, Agilent supplied consumables including:
• Loading tips (5067-5598; 5067-5599)
• Tube strips (401428)
• Tube strip caps (401425)
• 96-well sample plates (5042-8502)
• Robotics are calibrated to dimensions of the validated consumables. Using
other consumables may lead to either blockage errors or insufficient
volume being drawn by the instrument pump.
• To run samples from a 96-well sample plate, also use the Agilent seal foil
(5067-5154) to prevent the sample from leaving the plate during vortexing.
• Tips used are designed to be able to pierce the validated Agilent seal foil
(5067-5154). Using third-party seal foils may lead to either blockage errors
or insufficient volume being drawn by the instrument pump.
• After vortexing, use an appropriate centrifuge for either 96-well sample
plates, or tube strips to ensure that all of the samples are at the bottom of
the tube before placing in the TapeStation instrument.
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