Agilent 4150 TapeStation System Manual 101
Good Measurement Practices
7
Peak Integration
Peak Integration
In DNA assays, concentration values are calculated using the area of the
sample peak compared to the known concentration of the upper marker.
The user must ensure that both marker and sample peaks are properly
integrated, by manually adjusting the peak when necessary. An example of
correct upper marker peak integration is shown below.
Figure 27 Correct upper marker integration for DNA ScreenTape assays. Figure shows
D1000 ScreenTape assay.
In assays with no upper marker (RNA, High Sensitivity RNA, Genomic DNA, and Cell-free
DNA assay) quantification is calculated using the lower marker.