86 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
Reagents and Reagent Mixes
Reagents and Reagent Mixes
• Handle and store all reagents according to the instructions given in the
appropriate Quick Guide (Table 3 on page 64).
• Keep all reagents at the directed temperature when not in use. Reagents
that are left at room temperature for a long period of time may decompose,
leading to poor results.
• Allow all reagents to equilibrate to room temperature for 30 min. Mix and
spin down prior to use.
• When pipetting sample buffer, ensure that excess buffer droplets are
removed from the tip before transfer to the sample tubes. Care must be
taken due to viscosity of sample buffers.
• When adding sample buffer to samples, please ensure that they are mixed
correctly. See “Mixing Recommendations” on page 94, “Good Pipetting
Practice” on page 85, and the appropriate Quick Guide (Table 3 on page 64).
• When pipetting small volumes ensure that no sample remains within the
tip. Special care must be taken with high sensitivity assays which use small
volumes of sample buffer.
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