Agilent Technologies 4150 TapeStation system User Manual

198 Agilent 4150 TapeStation System Manual
10 Troubleshooting
Troubleshooting DNA Applications
TapeStation Analysis in the Agilent SureSelect and HaloPlex
Workflow
The TapeStation system has been verified for use within the Agilent
SureSelect and HaloPlex protocols.
• During the purification step however, residual AMPure XP beads can result
in a signal which runs with the upper marker peak.
• Any additional signal under the upper marker peak causes a lower
reported value of sample concentration.
• This artifact can be avoided by increasing the time for which the samples
are incubated on the magnetic plate to 10 minutes, thereby removing a
higher percentage of the beads.
Figure 49 Electropherogram image with additional signal from AMPure beads
above the Upper Marker peak (arrow)

Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English