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Agilent Technologies 4150 TapeStation system - Rine Functional Range

Agilent Technologies 4150 TapeStation system
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112 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
RINe Functional Range
RIN
e
Functional Range
To achieve accurate RIN
e
scores the samples used must be within the RIN
e
functional range as stated in the respective assay Quick Guide. Samples with
concentrations exceeding the RIN
e
functional range can show altered
electropherogram traces. Obtained RIN
e
scores are significantly impacted by
this (Table 3 on page 64).
Figure 38 Effects of overconcentrated samples on run distance of the Lower Marker
and RIN
e
values in the High Sensitivity RNA ScreenTape assay in the Gel view
of the TapeStation Analysis software.

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