Agilent 4150 TapeStation System Manual 91
Good Measurement Practices
7
Sample Preparation
Sample Preparation
• Refer to the assay-specific Quick Guide for the recommended sizing and
concentration ranges. Using samples outside the stated ranges can affect
performance (Table 3 on page 64).
• For salt tolerance guidelines, please refer to the assay-specific Quick Guide.
Please ensure that sample buffers are below the maximum recommendation
(Table 3 on page 64).
• If frozen, allow all samples to become liquid. Mix and spin down liquid
samples before use.
• Always pipette reagents against the side of the sample tube.
• Ensure that no residual material is left on the outside of the tip.
• When adding sample buffer to sample, please ensure that they are mixed
correctly by following the instructions in the Quick Guide and “Good
Pipetting Practice” on page 85. Improper mixing can lead to quantification
errors, see “Mixing Recommendations” on page 94 (Table 3 on page 64).
• Once mixed, briefly centrifuge to collect the contents at the base of tubes.
Visually confirm that all liquid is collected at the bottom of the vials.
• If running samples using tube strips, remove the strip caps carefully and
ensure that all liquid is still at the bottom after removal. If droplets are
formed after removing the strip caps, shortly centrifuge the vials before
placing them inside the instrument.
• Dispose of used sample tube strips and tips in accordance to local safety
regulations.
For successful loading, the sample solution must be placed at the bottom of the tube or
well without any air-bubbles. The TapeStation systems load a sample from a minimum of
3 µL onto the ScreenTape device.
Lids on sample tubes
Failure to remove sample tube lids can cause damage to the TapeStation instrument
and impact the performance.
➔ Ensure that lids are removed from the sample tube strips before starting the run.
For successful sample analysis, 96-well sample plates must be covered by 96-well plate
foil seal (96 -well Plate Foil Seal, Pack of 100 foils (5067-5154)) to prevent evaporation. The
TapeStation system is capable of piercing through the foil, providing only Agilent approved
foils are used.
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