100 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
RNA Assays
General Recommendations When Working with RNA
When working with RNA samples, make sure to consider following
recommendations:
• Always follow the GLP-rules established in the laboratory
• Wear gloves all the times to prevent contamination with RNases
• Thaw RNA samples and ladder on ice and keep them on ice during sample
preparation
• Ensure all working areas and plastic ware are RNase free
Figure 26 Example of a degraded RNA sample in the High Sensitivity RNA assay
resulting in missing peak identification in the Gel View (Left) and
Electropherogram View (Right) of the TapeStation Analysis software.
Not following these general guidelines might result in RNA degradation and false data.
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