106 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
Sizing Range
Sizing Range
In order to achieve accurate results the samples used must be within the sizing
range as stated in the respective assay Quick Guide. Signal of sample merging
with either Upper and/or Lower Marker will not be taken into consideration in
concentration calculation. Additional sample overlaying with the marker will
alter the known concentration of the marker that is used for concentration
calculation. This will influence the overall quantification performance of this
lane (Table 3 on page 64).
Figure 32 Example of a sample within the sizing range of the High Sensitivity D1000
assay (Left) and a sample merging with the Upper Marker (Right) in the
Region view of the TapeStation Analysis software.
If applicable use an assay with a broader sizing range.
Figure 33 Example of a sample within the sizing range of the High Sensitivity D5000
assay (left) and the same sample merging with the upper marker (right) in the
High Sensitivity D1000 assay.