64 Agilent 4150 TapeStation System Manual
6
Operating Instructions
Sample Preparation
Sample Preparation
Prepare the samples according to the assay-specific Quick Guide. Printed
Quick Guides are part of system shipment (“Accessories Shipment List” on
page 224). Quick Guides in electronic format are part of the Agilent
Information Center which is installed along with your TapeStation software.
They provide analytical and physical specifications such as sizing range,
resolution, sensitivity, maximum sample buffer strength, quantitative range,
analysis time, and the assay operating procedure.
Please notice a documentation on “Good Measurement Practices” on page 81.
Find information on “Troubleshooting DNA Applications” on page 178 and
“Troubleshooting RNA Applications” on page 200.
Please notice additional documentation on assays in the chapter ScreenTape Assays in
the Agilent Information Center.
Table 3 ScreenTape Assay portfolio
ScreenTape Assay Quick Guide Number
Agilent D1000 ScreenTape Assay G2991-90031
Agilent High Sensitivity D1000 ScreenTape Assay G2991-90131
Agilent RNA ScreenTape Assay G2991-90021
Agilent High Sensitivity RNA ScreenTape Assay G2991-90121
Agilent Genomic DNA ScreenTape Assay G2991-90041
Agilent D5000 ScreenTape Assay G2991-90051
Agilent High Sensitivity D5000 ScreenTape Assay G2991-90151
Agilent Cell-free DNA ScreenTape Assay G2991-90060
The assay-specific Quick Guides contain important information on the ladder, see also
“Ladder Considerations” on page 92. Please read this section carefully.