Agilent 4150 TapeStation System Manual 183
Troubleshooting
10
Troubleshooting DNA Applications
Incorrect Sizing Results
Sizing calculation is facilitated by comparing the run distance of a sample
peak or smear to the run distances of ladder peaks which originate from an
electronic ladder or a known actual run ladder. Sizing information is obtained
after aligning run distances. Both Lower and Upper Marker have a defined
molecular weight and are required to be assigned correctly for this alignment.
Incorrect sizing results can have multiple causes. If after following the
recommendations below, your result files continue to show sizing
discrepancies, please contact your local support representative.
For best sizing precision and accuracy, the user should run the appropriate ladder with the
samples.
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