182 Agilent 4150 TapeStation System Manual
10
Troubleshooting
Troubleshooting DNA Applications
Incorrect pipetting
technique or pipette
calibration.
Incorrect pipetting technique or uncalibrated
pipettes can lead to poor pipetting performance
which in turn alter sample/buffer ratio in an
unpredictable manner.
See “Good Pipetting Practice” on page 85 for
more information or follow guidelines of the
respective pipette vendor.
Incorrect Marker
peaks picked up
Depending on the sample pattern, wrong peaks
can get assigned as marker if running very
close to the actual marker.
Use the Aligned button to detect the correct
markers. Compare to aligned/unaligned data of
good, for example the ladder, and questionable
samples, then assign correct markers by
right-clicking. See the Agilent Information
Center for more information. Ensure that all
sample peaks are within the recommended
sizing range for the application.
Table 6 DNA - Possible causes of incorrect quantification results in order of probability
Root cause Explanation Solution
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