Agilent Technologies 4150 TapeStation system User Manual

Agilent 4150 TapeStation System Manual 181
Troubleshooting
10
Troubleshooting DNA Applications
Sample and the
marker relevant for
quantification have
merged.
Additional sample overlaying with the marker
will alter the known concentration of the
marker that is used for concentration
calculation.
Ensure that your sample is within the
recommended sizing range for the applications,
see “Sizing Range” on page 106.
Contaminating beads can alter the run behavior
of the sample and can introduce artifacts, see
“TapeStation Analysis in the Agilent SureSelect
and HaloPlex Workflow” on page 198.
If using the Sure Select Protocol, ensure that all
AMPure beads are removed by increasing the
time that the sample is on the magnetic plate.
Sample not collected
at bottom of vial
Insufficient volume pipetted by the robotics and
applied to the lane may affect migration
behavior and alter the band shape.
Make sure to collect all liquid at the bottom of
the vial by centrifugation. See “Centrifugation
Recommendations” on page 97. Ensure that
after removing strip caps and inserting the tube
strips or 96-well sample plate into the
TapeStation instrument, liquid is still located at
the bottom of the well, and is not spilled.
Incorrect protocol
used
Differences exist between Standard and High
Sensitivity assay protocols.
Ensure that the correct sample protocol for the
application is used, see “Use the Correct
Protocol” on page 93.
Incorrect reagents
used
Reagents from different assays may differ in
dye and marker concentration. Furthermore
markers used are different and upper markers
may not be present.
Ensure that the correct reagent buffer for the
application is used, see “Use the Correct
Protocol” on page 93.
Sample prepared too
long before analysis
Evaporation or settling may alter the sample to
buffer ratio.
Ensure that your run is started immediately
after sample preparation.
The sample/buffer mix may evaporate over
time if a 96-well sample plate was used which
was not sealed with the recommended seal foil.
Always cover with the recommended foil seal
(PN 5067-5154) and ensure tight sealing
throughout the 96-well sample plate.
Table 6 DNA - Possible causes of incorrect quantification results in order of probability
Root cause Explanation Solution

Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English