81
Agilent 4150 TapeStation System Manual
Agilent Technologies
7
Good Measurement Practices
Use of Correct Tools 83
Tools and Handling 84
Good Pipetting Practice 85
Reagents and Reagent Mixes 86
ScreenTape Devices 87
Partially Used ScreenTape Devices 90
Sample Preparation 91
Ladder Considerations 92
Use the Correct Protocol 93
Mixing Recommendations 94
Centrifugation Recommendations 97
RNA Assays 99
Heat Denaturation 99
General Recommendations When Working with RNA 100
Peak Integration 101
Peak Maxima Versus Average Molecular Weight Sizing and
Molarity 104
Sizing Range 106
Quantitative range 107
Maximum Buffer Concentration in Sample 110
RINe Functional Range 112
This chapter summarizes information on good measurement practices for
ScreenTape assays.
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