Agilent 4150 TapeStation System Manual 97
Good Measurement Practices
7
Centrifugation Recommendations
Centrifugation Recommendations
Spinning down samples during sample preparation is required to collect all
liquid in one droplet for effective mixing, to collect liquids at the bottom of the
vial so that they can be picked up by the pipetting robotics of the TapeStation
system, and to remove trapped air bubbles at the bottom of the vial.
A centrifuge for tube strips and 96-well sample plates is required. This
centrifuge is not supplied with the system. A centrifuge with a 8-tube strip
rotor and a centrifuge with a rotor for 96-well sample plates can be used, as
well as devices with interchangeable rotors. A suitable centrifuge should allow
spinning at a centrifugal force of approximately 500 G and, for example, for
the duration of 1 min. The effectiveness of this step should be confirmed by a
visual check.
Improper or skipped centrifugation
Failure to spin down samples can cause empty or smeared lanes on the ScreenTape
device as no or insufficient sample volume is transferred. Subsequently, no data or
data of low quality are created.
➔ Ensure that the sample liquid persists bubble free at the bottom of the sample tube
or well.
For successful sample analysis in 96-well sample plates as well as from sample tube
strips, both must be centrifuged. A centrifuge with a 8-tube strip rotor and a centrifuge
with a rotor for 96-well sample plates can be used as well as devices with interchangeable
rotors.
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