96 Agilent 4150 TapeStation System Manual
7
Good Measurement Practices
Mixing Recommendations
Figure 23 Chart of reported concentrations for the ScreenTape reagent mixing tests,
using the D1000 ScreenTape assay.
The concentrations are expressed as a percentage of the nominal
concentration for the three mixing methods. Again, the green bar represents
the recommended protocol of vortex mixing using the IKA vortexer and
adaptor at 2000 rpm for 1 minute followed by brief centrifugation; blue -
pipette mixing only; red - no mixing.
The table lists the quantification values that are obtained from the
TapeStation Analysis software and the Agilent D1000 ScreenTape assay when
using the correct protocol (vortex mix followed by brief centrifugation) and
two incorrect mixing protocols (pipette mixing only and no mixing) as
illustrated in the figures above.
Table 4 ScreenTape reagent mixing tests
Measured concentration
(ng/µL)
Theoretical concentration
(ng/µL)
Vortex mixing then
centrifugation
68.5 70
Pipette mixing only 21.2 70
No mixing 6.5 70
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