196 Agilent 4150 TapeStation System Manual
10
Troubleshooting
Troubleshooting DNA Applications
Cell-Free DNA Assay
High EDTA Concentration in Sample
A high concentration of EDTA in the sample might result in a sizing offset.
This is caused by a decreased run distance of the lower marker. EDTA may be
a contaminant in the sample as a remnant from sample preparation. Please
refer to the maximum buffer concentration in sample in the Quick Guide
(Table 3 on page 64).
Figure 47 Effect of increasing EDTA concentration on lower marker run distance in
aligned (left) and unaligned (right) gel view in the TapeStation Analysis
software.
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