Agilent 4150 TapeStation System Manual 195
Troubleshooting
10
Troubleshooting DNA Applications
Low signal intensity
of sample and marker
peaks (both) or of
ladder peaks
Incorrect reagent
storage
Incorrect storage of
the reagents may
induce degradation of
the dye. This can lead
to a higher than
normal background
noise.
Follow the storage conditions that are
specified for the assay (see Tabl e 3 on
page 64).
Incorrect reagents
used
Components of High
Sensitivity Assay
Reagents are too
diluted to be properly
detected by Standard
Sensitivity Assays,
see “Reagents and
Reagent Mixes” on
page 86.
Use the correct protocol and consumables
Incorrect migration -
samples have not
reached the lower end
of the gel lane
Salt concentration High salt
concentrations can
cause short running
within the gel lane
which can
subsequently cause
incorrect
identification of Lower
marker peaks, see
“Maximum Buffer
Concentration in
Sample” on page 110.
Unalign the image using the Aligned button in
the TapeStation Analysis software. This will
show the true position of all peaks present,
see “Peak Integration” on page 101. Refer to
the salt tolerance guidelines for the assay (see
Ta bl e 3 on page 64).
Partial
electrophoresis failure
caused by bubbles at
gel-buffer interface
Air bubbles at the
gel-buffer buffer
interface may disrupt
current flow between
the electrodes.
Ensure ScreenTape device is flicked to remove
any bubbles before use. See the Agilent
Information Center and “ScreenTape
Devices” on page 87 for more information.
Table 10 DNA - probable causes for unexpected migration profile
Problem Likely cause Explanation Solution
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