Agilent Technologies 4150 TapeStation system User Manual

194 Agilent 4150 TapeStation System Manual
10 Troubleshooting
Troubleshooting DNA Applications
Low signal intensity
of sample or marker
peaks (one or other,
not both)
Insufficient Mixing Buffer and sample
was not sufficiently
vortexed. The dye
could not incorporate
completely into the
sample.
See “Mixing Recommendations” on page 94
and the Agilent Information Center for more
information.
Incorrect ScreenTape
device or reagent
storage
Incorrect storage (too
cold or too warm) may
accelerate aging of
the ScreenTape device
and reduce the shelf
life. Storing reagents
incorrectly may
induce degradation of
the dye. Both can lead
to a higher than
normal background
noise.
Follow the storage conditions that are
specified for the assay (see Tabl e 3 on
page 64).
Sample concentration
outside recommended
range for application
Linear concentration
estimation cannot be
achieved for samples
exceeding the limits
in the specification
table.
Either dilute or concentrate the sample until it
is within the recommended range for the
application as stated in the respective assay
Quick Guide; and then prepare a new run, see
“Quantitative range” on page 107. Use the
High Sensitivity assay if applicable.
Table 10 DNA - probable causes for unexpected migration profile
Problem Likely cause Explanation Solution

Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English