Agilent 4150 TapeStation System Manual 193
Troubleshooting
10
Troubleshooting DNA Applications
Higher than normal
background noise
Insufficient Mixing Buffer and sample
were not sufficiently
vortexed. The dye
could not incorporate
completely into the
sample.
See “Mixing Recommendations” on page 94
and the Agilent Information Center for more
information.
Incorrect ScreenTape
device or reagent
storage
Incorrect storage (too
cold or too warm) may
accelerate aging of
the ScreenTape device
and reduce the shelf
life. Storing reagents
incorrectly may
induce degradation of
the dye. Both can lead
to a higher than
normal background
noise.
Follow the storage conditions that are
specified for the assay (see Tabl e 3 on
page 64).
Table 10 DNA - probable causes for unexpected migration profile
Problem Likely cause Explanation Solution
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