Agilent Technologies 4150 TapeStation system User Manual

192 Agilent 4150 TapeStation System Manual
10 Troubleshooting
Troubleshooting DNA Applications
The upper and lower
markers appear
unusually intense in
the gel image.
This effect might
appear during normal
alignment and
scaling.
DNA lower markers
are normalized to
appear for all lanes
the same in the
electropherogram,
which can result in
sample peaks
appearing smaller
than expected.
To get an idea about raw fluorescence
intensities, unalign the image using the
Aligned button in the TapeStation Analysis
software.
Sample prepared too
long before analysis
Evaporation or
settling may alter the
sample to buffer ratio.
Ensure that your run is started immediately
after sample preparation.
Insufficient Mixing Buffer and sample
was not sufficiently
vortexed. The dye
could not incorporate
completely into the
sample.
See “Mixing Recommendations” on page 94
and the Agilent Information Center for more
information.
The sample analyzed
is too diluted
The sample
concentration is
below the limit of
detection.
Concentrate your sample until it is within the
recommended range for the application; and
then prepare a new run, see “Quantitative
range” on page 107. Use the High Sensitivity
assay if applicable.
Figure 46 Example of intense upper and lower markers seen in the blue gel image. Lower
markers are normalized in electropherogram view and appear identical in signal.
Table 10 DNA - probable causes for unexpected migration profile
Problem Likely cause Explanation Solution

Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English