Agilent 4150 TapeStation System Manual 199
Troubleshooting
10
Troubleshooting DNA Applications
• An additional peak within the library smear appears in the analysis file.
• This peak is due to a concentration-dependent stacking effect and
represents no contamination in the library.
• This peak fronting can occur in Low Input libraries with medium to low
concentrations.
• Sizing and quantification results obtained in the Region View is still
within the given specifications.
• For examples with expected results within an Agilent SureSelect or
HaloPlex workflow please see the demo files available in the TapeStation
Analysis software.
Figure 50 Electropherogram image with additional peak in a library smear (arrow)
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