4PCR Programs
Gradient Step
48 Agilent SureCycler 8800 Setup and User’s Guide
During the run, the instrument generates a temperature gradient based on
the values entered into the Left Side Temperature Gradient and Right
Side Temperature Gradient fields. The difference between the minimum
and maximum temperatures must not exceed 30°C.
Left Side
T
emperature
Gradient
E
nter the desired temperature for the left side of the thermal block.
Right Side
Temperature
Gradient
E
nter the desired temperature for the right side of the thermal block.
Hold for Enter the duration of the Gradient Step (units are minutes :
seconds).
Convert to
Tempera
ture Step
(optional)
Once the optimal annealing temperature has been determined, the
Gradient Step can be easily converted to a standard Temperature Step by
marking this check box and specifying in the drop- down which
temperature column yielded the best amplification results. The Gradient
Step will then automatically be converted to a standard Temperature Step
in which the temperature of the thermal block is uniform.
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