DRAFT
September 25, 2007 1:07 am, 4376782_Troubleshoot.fm
Appendix A How to Troubleshoot the Installation
Background Calibration Failure
133
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
4. View the Spectral plot for the peak(s). If the peak is:
• Visible – The optics of your instrument may be contaminated. Contact
Applied Biosystems as explained in “How to Obtain Support” on page x.
• Absent – The sample block(s) are contaminated. Decontaminate the sample
block(s) as explained in “Decontaminate the Sample Block(s)” on page 115.