Applied Biosystems StepOnePlus User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
146
replicate group A set of identical reactions in an experiment.
replicates Total number of identical reactions containing identical components and identical
volumes.
reporter Fluorescent dye used to detect amplification. If you are using TaqMan
®
reagents, the
reporter dye is attached to the 5′ end. If you are using SYBR
®
Green reagents, the
reporter dye is SYBR
®
Green dye.
reverse primer An oligonucleotide that flanks the 3′ end of the amplicon. The reverse primer and the
forward primer are used together in PCR reactions to amplify the target.
reverse
transcriptase
An enzyme that converts RNA to cDNA. Reverse transcriptase is added to the PCR
reaction to perform 1-step RT-PCR.
Rn See normalized reporter (Rn).
ROX
™
dye A dye supplied by Applied Biosystems and precalibrated on the StepOne
™
and
StepOnePlus
™
systems. ROX dye is used as the passive reference.
rs number See refSNP ID.
run method Definition of the reaction volume and the thermal profile for the StepOne
™
or
StepOnePlus
™
instrument run.
sample The template that you are testing.
Sample DNA (10✕) In the StepOne™ software, a reaction component displayed on the Reaction Mix
Calculations tab of the Reaction Setup screen. The software assumes the sample DNA is
added to the reaction mix at a 10✕ concentration. For example, if the reaction volume is
20 µL, the calculated volume of sample for 1 reaction is 2 µL.
Sample Library In the StepOne
™
software, a collection of samples. The Sample Library contains the
sample name and the sample color.
Sample or Standard
(10✕)
In the StepOne
™
software, a reaction component displayed on the Reaction Mix
Calculations tab of the Reaction Setup screen. The software assumes the sample or
standard is added to the reaction mix at a 10✕ concentration. For example, if the reaction
volume is 20 µL, the calculated volume of sample or standard for 1 reaction is 2 µL.
sample/SNP assay
reaction
In genotyping experiments, the combination of which sample to test and which SNP assay
to perform in one PCR reaction. Each PCR reaction can contain only one sample and one
SNP assay.
sample/target
reaction
In quantitation experiments, the combination of which sample to test and which target to
detect and quantify in one PCR reaction. In the Design Wizard, you can detect and
quantify only one target in one PCR reaction. Use Advanced Setup to detect and quantify
more than one target in one PCR reaction.

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English